Fig. 7: Deletion of Utrn abrogates beneficial effects of Sirt6 inactivation in Sirt6mKO/mdx mice. | Nature Communications

Fig. 7: Deletion of Utrn abrogates beneficial effects of Sirt6 inactivation in Sirt6mKO/mdx mice.

From: Inactivation of Sirt6 ameliorates muscular dystrophy in mdx mice by releasing suppression of utrophin expression

Fig. 7

a Serum creatine kinase (CK) enzyme activity relative to mdx (n = 8), Sirt6mKO/mdx (n = 8), and Sirt6mKO/Utrn−/−/mdx (n = 8) mice (One-way ANOVA with Benjamini multiple comparisons test: *p = 0.0229). b Evans blue staining of diaphragm muscles from mdx (n = 3), Sirt6mKO/mdx (n = 4), Utrn−/−/mdx (n = 2) and Sirt6mKO/Utrn−/−/mdx (n = 4) mice. Cross-sectional images showing myofiber leakage in red color are shown. Scale bar: 100 μm. c Cumulative survival curve of Utrn−/−/mdx (n = 11) and Sirt6mKO/utrn−/−/mdx mice (n = 11). Kaplan–Meier curve and Log-rank test. d RT-qPCR analysis of expression of genes involved in cellular stress responses in control (n = 4), Utrn−/−/mdx (n = 4), and Sirt6mKO/Utrn−/−/mdx (n = 5) muscles. m36b4 was used as a reference gene (One-way ANOVA with Benjamini multiple comparisons test: Dab2: *p = 0.0259, *p = 0.0265; Hspa2: *p = 0.0437, *p = 0.0280; Ptpn22: *p = 0.0034, *p = 0.0290). Five to eight weeks old male and female mice were used. Data are presented as mean ± SEM. Source data are provided in the Source Data file.

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