Fig. 7: Co-localization of SCRIB and AFDN is dependent on their PDZ1 and FHA domains, respectively.
From: Afadin couples RAS GTPases to the polarity rheostat Scribble
a Expression of EGFP-AFDN in AFDN KO MCF7 cells restores endogenous SCRIB localization at cell contacts. SCRIB is also recruited to cell contacts in AFDN KO cells expressing EGFP-AFDNΔFHA but appears poorly co-localized with AFDN. Projections of z-stacks are at right and top and their position is marked with a dashed yellow line in the merged image. Scale bars represent 10 µm. b EGFP-SCRIB co-localizes with endogenous AFDN in SCRIB KO MCF7 cells. EGFP-SCRIBΔPDZ1 is also enriched at cell contacts in SCRIB KO cells. Projections of z-stacks are at right and top and their position is marked with a dashed yellow line in the merged image. Scale bars represent 10 µm. c z-stack projections along a 10 µm length of cell contacts show exogenous, wild-type AFDN and endogenous SCRIB localization overlaps in AFDN KO cells (top, 3 independent cells). Conversely, expressed AFDNΔFHA remains apical to endogenous SCRIB (bottom). d z-stack projections show exogenous, wild-type SCRIB and endogenous AFDN co-localize in SCRIB KO cells (top) while SCRIBΔPDZ1 remains basal to endogenous AFDN (bottom).
