Fig. 3: Two motifs in S1’ pocket determine Met1 linkage selectivity of OsOTUB1. | Nature Communications

Fig. 3: Two motifs in S1’ pocket determine Met1 linkage selectivity of OsOTUB1.

From: Met1-specific motifs conserved in OTUB subfamily of green plants enable rice OTUB1 to hydrolyse Met1 ubiquitin chains

Fig. 3

a Structural alignment between C-handle motifs of OsOTUB1-cat~Met1-diUb-DHA and hOTUB1-cat; b Structural alignment between N-handle motifs of OsOTUB1-cat~Met1-diUb-DHA and hOTUB1-cat; c Effects of mutations in two motifs on the hydrolysis activity of OsOTUB1-cat against Met1-diUb (N = 3 biologically independent experiments); d Effects of mutations in two motifs on the hydrolysis activity of hOTUB1-cat against Met1-diUb (N = 3 biologically independent experiments); e Detail interaction between N-handle motif of OsOTUB1 and proximal Ub; f Effects of mutations in N-handle motif on the hydrolysis activity against Met1-diUb (N = 3 biologically independent experiments). Source data are provided as a Source Data file.

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