Fig. 5: Depleting B cells ameliorated EAU and PIM1 regulated PCs differentiation.
From: Multicellular immune dynamics implicate PIM1 as a potential therapeutic target for uveitis
a t-SNE plots of B cell subsets from all mice groups. b Proportion of B cell subsets. c Frequency of PCs. Each group contains six mice. P(D0-D7) = 0.0316, P(D0-D14) = 7.7E-05, P(D0-D21) = 0.0047, P(D7-D14) = 0.0157. Data represented as mean ± SEM. Significance was determined using one-way ANOVA. *P < 0.05, **P < 0.01, ****P < 0.0001. d Percentage of BCR isotype. e Concentration of IRBP1-20-specific antibodies. Each group contains six mice. P(IgG, D0-D14) = 1.8E-07, P(IgG, D7-D14) = 1.2E-07, P(IgG,D14-D21) = 1.4E-06, P(IgG1, D0-D14) = 1.6E-08, P(IgG1, D7-D14) = 1.7E-08, P(IgG1,D14-D21) = 4.1E-06, P(IgG2b, D0-D14) = 6.2E-05, P(IgG2b, D7-D14) = 5.1E-05, P(IgG2b,D14-D21) = 0.0003. Data represented as mean ± SEM. Significance was determined using one-way ANOVA. ***P < 0.001, ****P < 0.0001. f Administration of anti-CD20 antibodies to deplete the B cells before (7 days before immunization, day −7) and after EAU (7 days after immunization, day +7). Representative fundus images of eyes after immunization at day 14. White arrowheads indicate inflammatory exudation. g Clinical scores after immunization at day 14. Each group contains six mice. P(D5, EAU- Anti-CD20 (day-7)) = 0.0493, P(D14, EAU- Anti-CD20 (day-7)) = 0.2557, P(D14, EAU- Anti-CD20 (day + 7)) = 6.0E-06, P(D21, EAU- Anti-CD20 (day-7)) = 0.7900, P(D21, EAU- Anti-CD20 (day + 7)) = 1.7E-06. Data represented as mean ± SEM. Significance was determined using one-way ANOVA. ns, no significant differences, *P < 0.05, ****P < 0.0001. h t-SNE plots of Pim1 by B cell subsets. i Violin plots of Pim1 in B cell subsets from all mice groups. j Proportions of PCs after immunization at day 14. Each group contains six mice. P(EAU-SMI-4a) = 0.0114. Data expressed as mean ± SEM. Significance was determined using unpaired two-tailed student’s t test. *P < 0.05. k Proportions of PIM1+ cells in PCs after immunization at day 14. Each group contains six mice. P(EAU-SMI-4a) = 1.7E-06. Data expressed as mean ± SEM. Significance was determined using unpaired two-tailed student’s t test. ****P < 0.0001. l Concentration of IRBP1-20-specific antibodies. Each group contains six mice. P(IgG) = 0.0207, P(IgG1) = 0.0027, P(IgG2b) = 0.0058. Data expressed as mean ± SEM. Significance was determined using unpaired two-tailed student’s t test. *P < 0.05, **P < 0.01. m Proportion of PCs. Data represented as mean ± SEM from six independent experiments. P(SMI-4a (-)-SMI-4a (+)) = 8.4E-05. Significance was determined using unpaired two-tailed student’s t test. ****P < 0.0001.
