Fig. 2: Vaccination is associated with increases in cytotoxic CD8 T cells and antigen presenting monocytes. | Nature Communications

Fig. 2: Vaccination is associated with increases in cytotoxic CD8 T cells and antigen presenting monocytes.

From: A phase I study of an adenoviral vector delivering a MUC1/CD40-ligand fusion protein in patients with advanced adenocarcinoma

Fig. 2

Mass cytometry data was clustered by FlowSOM and analysed for clusters that differed pre (red) and post (black)-vaccination. a Cluster phenotypes identified using a T cell focused panel (P1). b Manually gated cytotoxic CD8 T cells. Pre-vaccination: n = 12 patients, post-vaccination: n = 12 c Cluster phenotypes identified using a B cell/APC focused panel (P2). d Manually gated clusters in (c) Pre-vaccination: n = 15, post-vaccination: n = 14. Heatmap represents the scaled arcsin median expression of each marker. P-values were computed by Mann–Whitney U test (two-tailed). Error bars indicate SE of mean. For each staining panel, some samples were excluded from supervised analyses due to insufficient cell numbers. Source data are provided as a Source Data file.

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