Fig. 1: Single-cell transcriptomic atlas of human liver cells.
From: Unique DUOX2+ACE2+ small cholangiocytes are pathogenic targets for primary biliary cholangitis
a The strategies for preparing single liver cells for single-cell RNA sequencing (scRNA-seq). b UMAP analysis of 70,050 liver cells from four control (CTR) and five untreated primary biliary cholangitis (PBC) patients identidfied 30 distinct cell clusters. c Cluster distribution of cells in CTR livers (orange) and PBC livers (blue). d UMAP plot visualizing the distribution of identified 11 liver cell lineages. e Violin plot showing the expression levels of well-known representative enriched marker genes across 11 cell lineages, colored by cell lineage. f Heatmap displayed the relative expression level of marker genes in 11 cell lineages (top, color-coded by cell lineages), with exemplar genes labeled (right). Columns denote cells; rows denote genes. g The relative percentage of different cell lineages for each group, colored by cell lineage. CTR: n = 4 liver samples from CTR patients, PBC: n = 5 liver samples from PBC patients. h Comparision of the proportions of different cell lineages between CTR livers and PBC livers. CTR: n = 4 liver samples from CTR patients, PBC: n = 5 liver samples from PBC patients; Plotted: mean ± SEM; Statistics: two-tailed Mann–Whitney U test, 95% confidence interval; *p < 0.05. Source data are provided as a Source Data file.
