Fig. 4: Mice with TRAF3 deleted in MPCs have increased numbers of TGFβ-expressing neutrophils in BM.

a WBs of TRAF3, RelB, p52, and GAPDH expression in total protein lysate extracted from leg bones (with BM) from 3- (young) and 18-mon-old (aged) C57 mice. b von-Kossa-stained plastic sections of L1 vertebrae from 12-mon-old Prx1^Cre;TRAF3^fl/fl (P-cKO) mice and WT littermates. Bar = 400 μm. c H&E-stained paraffin sections of L2 vertebrae from 12-mon-old WT and P-cKO mice, and d OB surfaces (Ob.S/B.S). Bar = 400 μm in (c) and 50 μm in (d). Mean ± SD (n = 8 biologically independent male mice/group). e TRAP-stained paraffin sections of L2 vertebrae and OC numbers (Oc.N/B.S). Bar = 50 μm. Mean ± SD (n = 8 biologically independent male mice/group). f, g Frequencies and numbers of TGFβ1⁺CD11b⁺Ly6C^hi6G^- and TGFβ1⁺CD11b⁺Ly6C^-6G⁺ in BM from 15-mon-old WT and P-cKO mice. Mean ± SD (n = 5 biologically independent male mice/group). Analyses: Student’s two-sided unpaired t test. Source data are provided as a Source data file.