Fig. 5: Oxidation-damaged cell targeting ability and ROS scavenging capacity of C-dot SOD nanozymes.
From: Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme
a Illustration of the selective targeting and ROS scavenging ability of C-dot SOD nanozymes to oxidation-damaged cells, adapted from “Compare and Contrast Layout—Cell”, by BioRender.com (2023). Retrieved from https://app.biorender.com/biorender-templates. b Representative confocal imaging of C-dot SOD nanozymes accumulating in SH-SY5Y cells in the presence of various concentrations of H2O2 (n = 3 independent experiments). c Flow cytometry analysis and (e) corresponding quantification analysis of mean fluorescence density for the accumulation of C-dot SOD nanozyme in SH-SY5Y cells (n = 3 independent experiments). d Confocal images of the colocalization of Cy5.5 labeled C-dot SOD nanozymes (magenta) with mitochondria (yellow), and nuclei stained with DAPI (cyan) (n = 3 independent experiments). f Confocal images of the colocalization of Cy5.5 labeled C-dot SOD nanozymes (magenta) with lysosome (yellow), and nuclei stained with DAPI (blue) (n = 3 independent experiments). g ROS production detected by fluorescence probe DCFH-DA in SH-SY5Y cells using confocal microscopy. h Flow cytometry analysis and (i) corresponding quantification analysis of ROS levels in cells with different treatments (n = 3 independent experiments). j O2•− concentration detected by fluorescence probe DHE in SH-SY5Y cells using confocal microscopy. k Flow cytometry analysis and (l) corresponding quantification analysis of O2•− levels in cells with different treatments (n = 3 independent experiments). In (e, i, and l), data are presented as means ± SD from 3 independent experiments. P values are determined with one-way ANOVA Tukey’s multiple comparisons test. Source data are provided as a Source Data file. For (c, h, and k), the gate strategies were shown in Source Data.
