Fig. 2: METTL3-catalyzed m6A deposition mediates the degradation of m6A modified RNAs in GV oocytes. | Nature Communications

Fig. 2: METTL3-catalyzed m6A deposition mediates the degradation of m6A modified RNAs in GV oocytes.

From: scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development

Fig. 2

a Metagene profiles depicting m6A signals surrounding the stop codon in control and Mettl3 conditional knockout (cKO) GV oocytes. b Heatmap showing the METTL3-dependent m6A modified transcripts in GV oocytes. c Functional annotation analysis of METTL3-dependent m6A modified transcripts using Metascape. The enrichment and P value was calculated with default parameters using hypergeometric test of functional annotation in DAVID database. d Cumulative frequency of expression change (log2(fold change)) of METTL3-dependent, METTL3-independent m6A modified and unmodified RNAs upon Mettl3 silencing. P values were determined by one-sided Wilcoxon test. P = 1.2e−10, between METTL3-independent and W/O m6A RNA sets. P < 2.2e−16, between METTL3-dependent and W/O m6A RNA sets. ***P < 0.001. e Integrated Genomics Viewer (IGV) diagram displaying the translation signals of Ythdf2 in oocytes and zygotes. f Density plot displaying the distance between YTHDF2 target peaks detected by enhanced crosslinking and immunoprecipitation sequencing (eCLIP-seq) in mESCs42 (GSE151788) and METTL3-dependent m6A peaks in GV oocytes by scm6A-seq. g Integrated Genomics Viewer (IGV) diagram showing the METTL3-dependent m6A peaks across Cdc25b, Bag6, and B4galt5. The light blue boxes represent the identified m6A peaks identified by scm6A-seq.

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