Fig. 1: Inherent relationship between cfDNA end and size.
From: DNA methylation analysis explores the molecular basis of plasma cell-free DNA fragmentation
a Typical cfDNA size distribution from a healthy control subject. b Coverage and orientation-aware fragmentation end pattern in chr12p11.1 loci. Healthy controls, breast cancer patients (from Cristiano et al. dataset), and pregnant women (from Rabinowitz et al. dataset) were illustrated. For orientation-aware fragmentation end pattern, red and blue signals stand for upstream and downstream ends, respectively. c–f Orientation-aware fragmentation end distribution for short and long cfDNA in the nucleosomal context in chr12p11.1 loci: (c) healthy controls, (d) tumor-derived cfDNA in PDX model (using primary colon tumor), (e) breast cancer patients, and (f) pregnant women. g–i Genomewide orientation-aware cfDNA fragmentation end distribution for short and long reads in the nucleosomal context: (g) healthy controls, (h) breast cancer patients, and (i) pregnant women. Dashed lines in (c–i) indicated the border of nucleosome core (i.e., ±73 bp from nucleosome center) and nucleosome spacing (i.e., ±90 bp from nucleosome center).
