Fig. 2: Molecular recognition of INSL5 by RXFP4.
From: Ligand recognition mechanism of the human relaxin family peptide receptor 4 (RXFP4)
a Binding mode of INSL5 (green) with RXFP4 (orange), showing that the B chain of INSL5 (light green) penetrates into the transmembrane ___domain (TMD) bundle using the C-terminal α-helix, whereas the A chain of INSL5 (forest green) covers the orthosteric pocket mainly interacting with the extracellular loops 2 (ECL2, violet red) and 3 (ECL3, yellow). b Interactions of R23B and W24B with RXFP4 in the orthosteric pocket. c–e Close-up views of the interactions between the B chain of INSL5 and the residues in ECLs of RXFP4. f, g Effects of receptor mutations on INSL5-induced cAMP accumulation. Data are shown as means ± S.E.M. of at least three independent experiments. The numbers of independent experiments are shown in parentheses. Supplementary Table 4 provides detailed statistical evaluation such as P values and numbers of the independent experiment (n). Source data are provided as a Source Data file.
