Fig. 1: MORF and MOZ contain two DNA-binding WH domains.
From: MORF and MOZ acetyltransferases target unmethylated CpG islands through the winged helix ___domain
a Domain architecture of MORF with the MORFWH1, MORFWH2 and MORFDPF colored blue, green and wheat, respectively. b Overlay of 1H,15N HSQC spectra of 15N-labeled MORFWH1-WH2 (black), MORFWH1 (blue) and MORFWH2 (green). c, d EMSA of 147 bp 601 DNA in the presence of increasing amounts of His-MORFWH1 and MORFWH2. DNA:protein ratio is shown below the gel images. e Alignment of amino acid sequences of the N-terminal regions (1–200) of MORF and MOZ. Boundaries of WH1 and WH2 are indicated by arrows. f–h Anti-FLAG ChIP-qPCR analysis in K562 cells stably expressing WT, ΔWH1, ΔWH2, and ΔWH1-WH2 FLAG-MORFN (1-716), presented as IP/input % at the indicated genes (near TSS). ChIP analysis of H3K23ac (g) and H3K14ac (h) in K562 cells as in (f). Acetylation levels were corrected for nucleosome occupancy (total H3 signal), presented as a ratio of IP/input (H3ac/total H3). Data represent mean ± SEM from three independent experiments. n = 3 Statistical tests were two-sided. Student’s t-test, ***P < 0.005, 0.005 < **P < 0.01, and 0.01 < *P < 0.05. Source data are provided as a Source Data file.
