Fig. 5: CpG recognition is conserved in MOZ/MORFWH1.
From: MORF and MOZ acetyltransferases target unmethylated CpG islands through the winged helix ___domain
a Representative images of genomic localization of endogenous proteins and the indicated FLAG-tagged MOZ proteins. FLAG-tagged MOZ proteins were transiently expressed in HEK293T cells and analyzed by ChIP-seq. ChIP/CIRA-seq data at the HOXA, MYC, CDKN2C, and CD4 genes are visualized using the Integrative Genomics Viewer (The Broad Institute). ChIP-seq analysis for MOZWH1 was performed in two independent replicates. The data range for each sample is shown on the right. b Average distribution of MOZ WH1 and MORF WH1 near the TSSs. FLAG-tagged MOZ WH1 and MORF WH1 were transiently expressed in HEK293T cells and analyzed by ChIP-seq as in Fig. 4a. c Representative images of genomic localization of the FLAG-tagged MOZ WH1 and MORF WH1. ChIP-seq analysis for MORF WH1 was performed in two independent replicates. d Occupancy of the indicated wild type and mutated MORF WH1. ChIP-qPCR was performed for the CD4, MYC and HOXA9 gene loci using qPCR probes designed for the pre-TSS (−1 to −0.5 kb of TSS), TSS (0 to +0.5 kb of TSS) and post-TSS regions (+1 to +1.5 kb of TSS) of each gene. ChIP signals are expressed as a percentage of input (mean ± SD of technical replicates; n = 3). Source data are provided as a Source Data file.
