Fig. 9: Impact of auto-acetylation and the DNA linker on the HAT activity and binding to DNA.
From: MORF and MOZ acetyltransferases target unmethylated CpG islands through the winged helix ___domain
a In vitro liquid HAT assays show acetyltransferase activity of native WT and mutated MORFN complexes, purified from K562 cells on NCP207 and NCP147. Values for NCP207 are normalized to the corresponding values for NCP147. Data represent mean ± SEM from three independent experiments. n = 3 Statistical tests were two-sided. Student’s t‐test, ***P < 0.005, 0.005 < **P < 0.01 and 0.01 < *P < 0.05. b Acetylated MORFWH2-DPF-MYST sequences detected by MS. c–e EMSAs of 147 bp DNA in the presence of increasing amounts of WT or mutated MORFWH2-DPF. DNA:protein ratio is shown below the gel images. f Quantitative densitometry analysis of the DNA147 band in c–e. g EMSAs of NCP147 and NCP187 in the presence of increasing amounts of MORFWH2-DPF. NCP:protein ratio is shown below the gel images. h Quantitative densitometry analysis of the NCP147 and NCP187 bands in g. i A model for the interaction of MORFWH1-WH2-DPF-MYST with the nucleosome. Source data are provided as a Source Data file.
