Fig. 4: Comprehensive study of DNA base stacking.
From: High-throughput single-molecule quantification of individual base stacking energies in nucleic acids
a Decay curves and single-exponential fits obtained for unique base stacking combinations (blue) and their controls (gray) at a constant force of 15 pN. b Off-rates of DNA tethers containing various base stacks and their corresponding controls. c Base stacking energies calculated from panel (b). Data in (a, b) presented as mean values +/− standard deviation from three independent data sets (shown in Supplementary Figs. 7–9). Data in (c) presented as calculated value from mean +/− propagated errors from (b). n represents the number of individual molecular tethers.
