Fig. 2: CD4⁺-cell depletion in tissues at necropsy.

a Percentage of CD4⁺ T-cells among CD3⁺ T-cells in different tissues collected at necropsy in CD4⁺-cell-depleted (blue circles) and control (yellow squares) AGMs. b Magnitude of CD4⁺ T-cell depletion in the tissues of CD4⁺-cell-depleted AGMs was evaluated by dividing the percentage of CD4⁺ T-cells among CD3⁺ T-cells in a specific tissue by the mean CD4⁺ T-cell percentage among CD3⁺ T-cells for the same tissue in the control group. For (a) and (b), bars represent the means (n = 6 AGMs for both groups). Data were analyzed with unpaired t tests with Welch correction using the Holm-Šídák method for multiple comparisons. Asterisks indicate statistical significance, with *p < 0.05; **p < 0.01 and ***p < 0.001. c–f Representative images showing IHC staining for CD4 expression (brown) in the GI tract of controls (c, d) and CD4⁺-cell-depleted (e, f) AGMs. Images of Peyer’s patches (c, e) and lamina propria (d, f) are shown. On all IHC images, scale bar is 50 μm. These pictures are representative of the 6 CD4⁺-cell-depleted AGMs.