Fig. 6: T_RM cells showed the presence and expansion of unique T cell clones.

a Schematic of TCRα/β deep sequencing of CD8⁺ T cells from the brains and draining lymph nodes (dLNs) of T-αFGL2-treated survivors. Cells were sorted via flow cytometry on day 20 after the third challenge with intracranially (i.c.) injected DBT tumor cells. b Representative tree maps (top row) of TCRα-T_RM -CD8⁺T, TCRβ -T_RM -CD8⁺T, and TCRβ-dLNs-CD8⁺T cell clones. Each spot represents a unique entry: V-J-CDR3, and the size of a spot denotes its relative frequency; 2D map of V and J usage of TCRα-T_RM -CD8⁺T, TCRβ-T_RM -CD8⁺T, and TCRβ-dLNs-CD8⁺T cell clones (bottom row). The experiments were repeated twice with similar results. c, Schematic of experimental design. On day 1, 3 × 10⁴ CD8⁺T_RM cells and 3 × 10³ DBT cells were coinoculated i.c. into naïve Balb/c mice; on days 0, 5, 10, 15, 20, and 25, the mice were treated with IgG or MHC-I blocking antibodies (100 µg/mouse, i.p.). d Representative bioluminescence images of Balb/c mice on days 0, 14, and 28 after i.c. coinoculation with CD8⁺T_RM and DBT cells. e Kaplan–Meier survival curves of mice in (f) (n = 4 mice/group), log-rank test. The experiments were carried out twice with similar results. f Representative H&E staining of brains from (d) collected on day 40 after transplantation. Similar observations were made in three mice per group and representative images are shown.