Fig. 3: Genetic determinants of pseudoiodinine biosynthesis. | Nature Communications

Fig. 3: Genetic determinants of pseudoiodinine biosynthesis.

From: The natural pyrazolotriazine pseudoiodinine from Pseudomonas mosselii 923 inhibits plant bacterial and fungal pathogens

Fig. 3

a Mutant 34–6 of P. mosselii 923 lacks antimicrobial activity for Xoo PXO99A and contains a Tn5 insertion in gacA. b Antibacterial activity assays of P. mosselii 923, ΔpsdA, ΔpsdB, ΔpsdC, ΔpsdD, ΔpsdE, ΔpsdF and ΔpsdG mutants and their corresponding complemented strains (CΔpsdA - CΔpsdG). c HPLC analysis (Method B, detected at 500 nm) of mutants ΔpsdA, ΔpsdB, ΔpsdC, ΔpsdD, ΔpsdE, ΔpsdF, and ΔpsdG. d HPLC analysis (Method B, detected at 500 nm) of complemented mutants CΔpsdA - CΔpsdG. e Schematic of the pseudoiodinine biosynthetic gene cluster in P. mosselii 923. Numbers above the gene names indicate the ORF length. Colors denote gene functions as follows: red, primary biosynthetic genes; gray, additional biosynthetic genes. The seven gene annotations are displayed as follows: psdA, methyltransferase type 12; psdB, glyoxylase bleomycin resistance protein dioxygenase superfamily protein; psdC, sulphatase-modifying factor protein; psdD, GTP cyclohydrolase; psdE, WD-40 repeat protein; psdF, methyltransferase ___domain; psdG, riboflavin biosynthesis protein RibD.

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