Fig. 1: Tissue retinoid concentrations and survival of mice during LPS-induced ALI. | Nature Communications

Fig. 1: Tissue retinoid concentrations and survival of mice during LPS-induced ALI.

From: Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice

Fig. 1

a Total retinyl ester concentrations (nmol/g) in lungs and liver determined by HPLC in mice 7 days after intranasal instillation of LPS (25 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c, d) are statistically different (a is different from b, p = 1.80e-06; c is different from d, p = 1.67e-05). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. b Retinol concentrations (nmol/g) in lungs and liver determined by HPLC in mice 7 days after intranasal instillation of LPS (25 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c, d) are statistically different (a is different from b, p = 0.0014; c is different from d, p = 1.92e-08). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. c Plasma retinol concentrations (µM) determined by HPLC in mice 7 days after intranasal instillation of LPS (25 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b) are statistically different (a is different from b, p = 1.91e-07). Statistical differences were analyzed by a one-way ANOVA. All values are given as the mean ± 1 S.D., n = 10 for each group. d Quantitative representation of retinol-binding protein 4 (Rbp4) concentrations determined by immunoblot (upper insert) in plasma of mice 7 days after intranasal instillation of LPS (25 mg/kg of body weight) or vehicle. Each lane represents an extract prepared from an individual animal. Values marked with different letters (a, b) are statistically different (a is different from b, p = 6.23e-05). Statistical differences were analyzed by a one-way ANOVA. All values are given as the mean ± 1 S.D., n = 6 for each group. e, f Kaplan–Meier survival curves for Lrat−/− and Rbp4−/− mice as well as for their respective littermate controls (Lrat+/+ (e) and Rbp4+/+ (f)) over the 7-day period after intranasal instillation of a LPS (25 mg/kg of body weight in PBS) or PBS alone. A statistically significant difference (p = 0.0004) was detected using the log rank test comparing the survival curves for LPS-instilled Lrat+/+ and Lrat−/− mice, n = 14 for each group.

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