Fig. 5: Lung functional parameters in mice during LPS-induced inflammatory stress. | Nature Communications

Fig. 5: Lung functional parameters in mice during LPS-induced inflammatory stress.

From: Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice

Fig. 5

a Bronchoalveolar lavage (BAL) fluid cell numbers in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 0.0025; a is different from c, p = 6.23e-12; b is different from c, p = 3.63e-08). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each Lrat+/+ group and n = 9 for each Lrat−/− group. b Tnfα concentrations determined by ELISA in cell-free BAL fluid of mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight) or PBS. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 0.0005; b is different from c, p = 3.63e-11; b is different from c, p = 4.81e-07). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 8 for each group. c Tnfα concentrations determined by ELISA in plasma of mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight) or PBS. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 0.025; a is different from c, p = 1.42e-06; b is different from c, p = 0.0009). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 8 for each group. d Extravascular lung water (EVLW) for mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight) or PBS. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 1.82e-10; a is different from c, p = 2.13e-14; b is different from c, p = 0.0014). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. e Whole lung Sftpb mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 0.0077; a is different from c, p = 9.15e-07; b is different from c, p = 0.0039). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. f Whole lung Sftpc mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b) are statistically different (a is different from b, p = 0.0015). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. g Heatmap indicating relative concentrations of BAL lipids determined by UPLC-MS/MS in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. h BAL concentrations of dipalmitoylphosphatidylcholine (PC 32:0) determined by UPLC-MS/MS in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 1.21e-05; a is different from c, p = 1.43e-06; b is different from c, p = 0.023). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 5 for each group. i BAL concentrations of phosphatidylglycerol (PG 34:1 + PG 34:2) determined by UPLC-MS/MS in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 8.21e-05; a is different from c, p = 1.43e-05; b is different from c, p = 0.0048). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 5 for each group. j Whole lung Cdh1 mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b) are statistically different (a is different from b, p = 0.0005). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. k Whole lung Cdh5 mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 2.08e-07; a is different from c, p = 0.0052; b is different from c, p = 0.0016). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. l Whole lung Ocln mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 2.45e-06; b is different from c, p = 0.0006). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. m Whole lung Tjp1 mRNA expression determined by qRT-PCR in mice 24 h after intranasal instillation of LPS (5 mg/kg of body weight in PBS) or PBS alone. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 1.25e-07; a is different from c, p = 0.0023; b is different from c, p = 0.0023). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group. Values marked with different letters (a, b, c) are statistically different (a is different from b, p = 2.45e-06; b is different from c, p = 0.0006). Statistical differences were first analyzed by a one-way ANOVA followed by multiple comparisons employing Tukey’s HSD post hoc test. All values are given as the mean ± 1 S.D., n = 10 for each group.

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