Fig. 2: Slc37a2 is a candidate regulator of human bone mass and is highly expressed in mature osteoclasts. | Nature Communications

Fig. 2: Slc37a2 is a candidate regulator of human bone mass and is highly expressed in mature osteoclasts.

From: Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts

Fig. 2

a CIRCOS plot71 displaying mouse chromosomes, gene names for the top 218 proteins up-regulated in the SL fractions, scatterplot representing protein abundance ratio (secretory lysososomes (SLs)/homogenate) adjusted P values (purple) with significant associations (P < 0.05) colored red, histogram representing Log2FC abundance values for each protein SLs/homogenate purple (+ve), green (−ve), gene groupings representing human homologs associated with eBMD at P < 6.6E−9 (green) and P < 5E−20 (red)23. Protein abundance ratio adjusted P values (ANOVA, Benjamini–Hochberg) are displayed as −Log10 P values. Red box denotes Slc37a2 and green boxes other notable genes. b Regional association plots for the human SLC37A2 gene (red box) generated using estimated bone mineral density (eBMD) GWAS association results23. Genetic variants within 100 kb of the lead variant (rs7949048, purple) are depicted (x-axis) along with their eBMD P value (–Log10) generated using a linear mixed non-infinitesimal model (LMM). c Slc37a2 expression in the femur of 12-week-old male WT mice by immunohistochemistry. Arrows indicate osteoclasts and a dashed line defines bone (white) and osteoclast (yellow) surfaces. Bars, 10 μm (n = 3). d Slc37a2 expression during in vitro osteoclast differentiation of bone marrow monocytes (BMMs) by immunoblotting. Cathepsin K (Ctsk) and β-actin served as controls (n = 3). Source data are provided as a Source Data file.

Back to article page