Fig. 5: Slc37a2^KO exhibits high bone mass and increased bone strength.

a Radiography of the hindlimbs of 12-week-old female WT and Slc37a2^KO mice. Bar, 1 mm. b–d Representative sagittal µCT section false-colored to indicate X-ray attenuation (b) and µCT 3D reconstructed images of sagittal (c) and transverse (d) sections of distal femurs of 12-week-old female WT and Slc37a2^KO mice. e–i µCT analysis of femurs of 12-week-old male and female WT (n = 8 male and 6 female) and Slc37a2^KO (n = 7 male and 6 female) mice. e Male P < 0.0001 and female P < 0.0001. f Male P < 0.0001 and female P < 0.0001. g Male P < 0.0001 and female P < 0.0001. h Male P = 0.7874, female P < 0.0001. i Male P = 0.0114, female P = 0.0053. j and k Biomechanical three-point bending test of 12-week-old male WT and Slc37a2^KO femurs. Stiffness (j, P = 0.0083) and ultimate force (k, P = 0.0013) are presented (n = 8 mice per genotype). l–n Representative µCT images of vertebrae (l) and µCT analysis of vertebrae (m and n) of 12-week-old male and female WT (n = 5 male and 8 female) and Slc37a2^KO (n = 6 male and 4 female) mice. m Male P < 0.0001 and female P < 0.0001, n male P < 0.0001 and female P < 0.0001. o–q Representative µCT images of female femurs (o) and µCT analysis of femurs (p and q) of 6-, 12- and 24-week-old male and female WT (6-weeks n = 4 male and 4 female; 12-weeks n = 8 male and 6 female; 24-weeks n = 7 male and 7 female) and Slc37a2^KO (6-weeks n = 4 male and 6 female; 12-weeks n = 7 male and 6 female; 24-weeks n = 8 male and 5 female) mice. p 6-weeks male P = 0.026112, female P = 0.004736; 12-weeks male P < 0.0001, female P < 0.0001; 24-weeks male P < 0.0001, female P < 0.0001). q 6-weeks male P = 0.014847, female P = 0.006968; 12-weeks male P < 0.0001, female P < 0.0001; 24-weeks male P < 0.0001, female P < 0.0001). All data are presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed unpaired Student’s t-test. Source data are available in the Source Data file.