Fig. 3: Comparison of imaging Riboglow-tagged RNA by fluorescence intensity vs. lifetime. | Nature Communications

Fig. 3: Comparison of imaging Riboglow-tagged RNA by fluorescence intensity vs. lifetime.

From: Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)

Fig. 3

Quantitative comparison of Riboglow-tagged RNA visualization via background-subtracted fluorescence intensity (a) and fluorescence lifetime imaging microscopy (FLIM) (b), as outlined in Fig. 2a. For each condition, the same cells were analyzed in (a) and (b). A whole-cell was defined as an ROI and fluorescence intensity (a) or average fluorescence lifetime (as defined in Fig. 2) (b) were extracted (6 independent experiments, 160 cells, 1 symbol = 1 cell). (ns: p ≤ 0.5; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001, see Supplementary Table 4). One-way ANOVA (95% confidence limit); post hoc test (Tukey HSD). Error bars indicate mean and standard deviation (+/−SD).

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