Fig. 5: The specific effect of nicotine on SIRT1-NAMPT interaction. | Nature Communications

Fig. 5: The specific effect of nicotine on SIRT1-NAMPT interaction.

From: Nicotine rebalances NAD+ homeostasis and improves aging-related symptoms in male mice by enhancing NAMPT activity

Fig. 5

a Nuclear SIRTUIN (SIRT1, SIRT6, SIRT7) activity of aged mice brain with or without nicotine at 13 months of age (n = 3, biologically independent samples/group). b Nicotine has no district effects on NAMPT-SIRT6/ SIRT7 interaction. c The total NAD+ levels of SIRT1 knockdown cells after nicotine treatment (n = 3, biologically independent samples/group). d Nicotine (10 ng/mL) has no district enhancement on SIRT1 interact with other substrates (p53, PGC-1, NF-κB). e The NAMPT inhibitor FK866 abolished the effect of nicotine (n = 3, biologically independent samples/group). f SIRT1 inhibitor has no district effect on SIRT1-NAMPT interaction. g The effect of SIRT1 activator SRT1720 (2.5 μg/mL) and inhibitor Selisistat (5 μmol/L) on SIRT1-NAMPT interaction. h Cotinine has no effect on SIRT1-NAMPT interaction. All western blotting images and graphs are representative of three independent experiments. Data are means ± SEM. p values were determined by two-sided Student’s t test (a), or one-way ANOVA with Tukey’s multiple comparisons tests (c, e).

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