Fig. 5: Abnormal H3K9me3 is incidental to incorrect H3.1/3.2 incorporation introduced by major satellite RNA. | Nature Communications

Fig. 5: Abnormal H3K9me3 is incidental to incorrect H3.1/3.2 incorporation introduced by major satellite RNA.

From: LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei

Fig. 5

a Immunostaining for H3.1/3.2 and H3.3 in representative pronucleus of Ctrl and MajSat_OE zygotes. Scale bars, 20 µm. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. b Scatterplot showing H3.1/3.2 and H3.3 fluorescence intensity mean values in pronucleus of Ctrl and MajSat_OE zygotes. The statistical data are expressed as mean ± SEM, *p < 0.05 and ns means not significant (H3.1/3.2: male p = 0.0438, female p = 0.6590, H3.3: male p = 0.0380, female p = 0.5885) by two-sided Student’s t test for each comparison. Each treatment contains 3 biological replicates, with each replicate containing at least 5 zygotes. c Immunostaining for H3K14ac in representative pronucleus of Ctrl and Lsm1_KD zygote. Scale bars, 20 µm. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. d Immunostaining for H3K14ac in representative pronucleus of Ctrl and MajSat_OE zygote. Scale bars, 20 µm. Representative images from 3 biological replicates, with each replicate containing at least 10 zygotes. e Scheme for the RNA pulldown to determine the interaction between MajSat RNA and H3.1/H3.3. f, g Pulldown of H3.1, H3.3 using intact and truncated MajSat RNA. Representative images from 3 biological replicates.

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