Fig. 3: McPGK1 drives the metabolic reprogramming from OXPHOS to glycolysis.
A, B Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of mcPGK1 silenced (A) and overexpressing (B) cells. Huh7 cells were used for mcPGK1 knockdown (A) and Hep3B cells were used for mcPGK1 overexpression (B). n = 3 independent experiments for each cell. C Intracellular levels of the indicated metabolites in mcPGK1 silenced (left, sample #2) and overexpressing (right, sample #4) panels. n = 4 independent experiments. D Five mcPGK1 high-expressing (mcPGK1high, sample #2, #3, #12, #10, #13) and mcPGK1 low-expressing (mcPGK1low, sample #4, #6, #7, #1, #15) samples were used for metabolite detection. E, F The abundance of lactate (E) and α-KG (F) in the indicated medium supernatant was measured at the indicated time points. Huh7 and Hep3B were used for mcPGK1 knockdown and overexpression. n = 4 independent experiments. G Acidification of the culture medium in mcPGK1 silenced (Huh7) and overexpressing (Hep3B) cells, as indicated by the color change of the phenol red indicator in the medium to orange/yellow. Typical images were shown representative of n = 3 independent experiments. In all panels, data are shown as mean + s.d. *P < 0.05; **P < 0.01; ***P < 0.001, by two-tailed Student’s T-test. Source data are provided as a Source Data file.
