Fig. 6: Topical LNA-anti-miR-200b induces emergence of vasculogenic fibroblasts in cutaneous wounds.
From: Identification of a physiologic vasculogenic fibroblast state to achieve tissue repair

a, b Immunofluorescence confocal image of day 7 wounds in Fsp1-Cre tdTomato mice treated with LNA control and LNA anti-miR 200b inhibitor showing lectin perfused Fsp1 positive vessels. Scale, 20 µm. The images showing the colocalization of lectin and Fsp1 in the 3D cross-sectional view rendered by Zen software (n = 6–7). c miR-200b expression in wound-edge tissue of Fsp1-Cre tdTomato mice treated with LNA control and LNA anti-miR 200b inhibitor at day 56 (n = 6–7). d Heat map showing gene expression in laser captured samples in Fsp1-Cre tdTomato mice treated with LNA control and LNA anti-miR 200b inhibitor at day 7 and day 56 (d7, n = 4; d56, n = 5) The red asterisks indicate the gene that were upregulated at day 7 and day 56. The red arrow indicates gene that were upregulated both in day 7 and day 56. *p < 0.05. Data expressed as mean ± S.D. Data in a–d were analyzed by two-tailed unpaired Student’s t test.