Fig. 7: Physiological relevance of the NET1ΔC-GAG interaction in the model system C. elegans (part 1). | Nature Communications

Fig. 7: Physiological relevance of the NET1ΔC-GAG interaction in the model system C. elegans (part 1).

From: The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation

Fig. 7

a Schematic representation of post-embryonic hermaphrodite gonad development. Phase 1 migration begins along the anteroposterior (AP) axes followed by phase 2 leading the gonad arm along the dorsoventral (DV) axes. Phase 3 represents cessation of migration. Leading distal tip cells (DTC) are indicated in blue. b Representative Differential Interference Contrast (DIC) micrograph of gonad arms of L4 stage wild type animals in lateral view (first panel). Class 1 represents the classic unc-5/unc-6 defect, classes 2–4 frequently observed defects during the indicated phase, class 5 mild or defects other than guidance defects. Gonad migration trajectories are indicated by black dotted lines. Scale bar: 20 µm. c Quantification of DTC migration defects of anterior gonad arms of wild type N2, NET1/unc-6(ev400) (null mutant), single and double mutants of NET1/unc-6 (loop cd) mutants ((unc-6(syb2327) & unc-6(syb2328)). Error bars represent mean ± SD. Results for ordinary 1-way ANOVA Tukey multiple comparisons test are indicated: ****, P < 0.0001; **, P < 0.0072; ns, not significant. Samples size for genotypes were N = 60 (crosses with unc-40(n324) and unc-5(e152)); N = 120 (crosses with HSPGs); N > 300 (wild type N2).

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