Fig. 8: Physiological relevance of the NET1ΔC-GAG interaction in the model system C. elegans (part 2). | Nature Communications

Fig. 8: Physiological relevance of the NET1ΔC-GAG interaction in the model system C. elegans (part 2).

From: The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation

Fig. 8

a Schematic representation of workflow for NET1/UNC-6 puncta scoring. b Representative confocal fluorescent images of NET1/UNC-6 puncta in the head region of day 1 and day 9 adult worms grown at 20 °C or 25 °C. Scale bar: 20 µm. c Quantification of number of NET1/UNC-6 puncta per animal in the head region of day 1 and day 9 adult worms grown at 20 °C or 25 °C. Mean with range and results for Kruskal–Wallis Dunn’s multiple comparisons test are indicated: ****, P < 0.0001; ***, P < 0.0010; *, P < 0.0472; ns, not significant. For each genotype and condition between N = 36 to N = 39 worms were analysed. d Quantification of size distribution of NET1/UNC-6 puncta per genotype in the head region of day 1 and day 9 adult worms grown at 20 °C or 25 °C. Median with range and results for Kruskal–Wallis Dunn’s multiple comparisons test are indicated: ****, P < 0.0001; ***, P < 0.0004; **, P < 0.0027; ns, not significant. For each genotype and condition between N = 36 to N = 39 worms were analysed.

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