Fig. 4: Transcriptomic analysis of the liver in Egr-1-deleted mice with age increased.

A Heatmap representation of the genes at Egr-1 highest and lowest zeitgeber time of mice aged 2 months, 6 months, and 12 months. The colors from blue to yellow indicate low to high gene expression levels, respectively. B Intersect gene counts statistics of Venn diagrams in Supplementary Fig. 4A. C Selected significantly enriched lipid-related GO terms of 2-month and 12-month intersect genes. The statistical test of data analysis was performed using a hypergeometric test, two-tailed, no adjustment (p < 0.05). D Venn diagrams representing the overlap between 2m_no_intersect_down and 12_intersect_down group. The lipid metabolism-related genes were labeled with red font. E Euclidean distance of each group difference at different ages. F Volcano plot displayed genes expressed in WT_H and KO_H groups in mice at the age of 6 months. The relative expression changes and significance levels are shown. The statistical test of data analysis was performed using two-tailed, no adjustment (p < 0.05) from the limma package in the R environment. G Selected significantly enriched GO terms. The x-axis and y-axis represent the enrichment and significance level, respectively, and the size of the circle represents the number of genes associated with the GO term. The statistical test of data analysis was performed using a hypergeometric test, two-tailed, no adjustment (p < 0.05). H Chord diagram revealing the enrichment levels of genes related to selected GO terms. I Relative FPKM values of Cidea in WT_H and KO_H group. We used four mice per group for the analysis. One-way ANOVA for A and unpaired two-tailed Student’s t-test for D, E, and G.