Fig. 5: Egr-1 regulates liver metabolic aging in a Cidea-dependent manner.

A mRNA levels of Cidea in samples from RNA-seq (n = 3 biologically independent animals per group); B Protein levels of Cidea in samples from RNA-seq; C Protein levels of Cidea in primary hepatocytes of WT and Egr-1 LKO mice at 6 months of age; D Oil Red O staining of primary hepatocytes of WT and Egr-1 LKO mice at 6 months of age; E Immunofluorescence staining of primary hepatocytes of WT and Egr-1 LKO mice at 6 months of age; F TG levels in WT and Egr-1 LKO primary hepatocytes from 2-month-old and 6-month-old mice after infection with a ShCidea adenovirus (n = 4 biologically independent samples); G Oil Red O staining of WT and Egr-1 LKO primary hepatocytes from 2-month-old and 6-month-old mice after infection with a ShCidea adenovirus; H Hepatocyte TG levels after transfection with a Cidea overexpression plasmid or infection with an Egr-1 overexpression adenovirus (n = 4 biologically independent samples); I Oil Red O staining after transfection with a Cidea overexpression plasmid or infection with an Egr-1 overexpression adenovirus; J the fatty acid uptake ratio after transfection with a Cidea overexpression plasmid or infection with an Egr-1 overexpression adenovirus (n = 14 biologically independent samples). Data were represented as mean ± SEM. Exact p values are depicted in the figure. Statistical analysis was performed using one-way ANOVA. Source data are provided as a Source Data file.