Fig. 3: Off-target assessments of hyper ABE and hyper A&C-BEmax.
From: Improving adenine and dual base editors through introduction of TadA-8e and Rad51DBD
a Cas9-dependent DNA on and off-target analysis of the indicated targets (HEK site 2 and FANCF site 1) by ABE8e, hyABE, A&C-BEmax, eA&C-BEmax and hyA&C-BEmax in HEK293T cells. Lowercase protospacer sequences represent mismatched bases compared to their corresponding on-target sequences. Data are means ± SD (n = 3 independent experiments). b, c Cas9-independent DNA off-target analysis of ABE8e, hyABE, A&C-BEmax, eA&C-BEmax, and hyA&C-BEmax using the modified orthogonal R-loop assay at each R-loop site with nSaCas9-sgRNA. Data are means ± SD (n = 3 independent experiments). d, e RNA off-target editing activity by ABE8e, hyABE, A&C-BEmax, eA&C-BEmax and hyA&C-BEmax using RNA-seq. Each biological replicate is listed on the bottom. For a–c, significance was tested by two-tailed Student’s t test. Source data are provided as a Source Data file.
