Fig. 6: The chromodomain of CEC-5 binds H3K9 peptides in vitro. | Nature Communications

Fig. 6: The chromodomain of CEC-5 binds H3K9 peptides in vitro.

From: Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans

Fig. 6

a Schematic diagram of the histone peptide pull-down assay. b Schematic diagram of the GST-tagged CEC-5 chromodomain (CEC-5 CD) and CEC-4 chromodomain (CEC-4 CD). c–f Coomassie blue-stained SDS‒PAGE gels showing binding of the indicated proteins to biotinylated histone H3 peptides. GST alone served as a negative control. The CEC-4 chromodomain was used as a positive control. c H3 (1-21) K9me0 (middle) and H3 (1-21) K9me1 (right). d H3 (1-21) K9me2 (middle) and H3 (1-21) K9me3 (right). e H3 (14-34) K27me0 (middle) and H3 (14-34) K27me3 (right). f no peptides (right). Source data are provided as a Source Data file.

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