Fig. 2: SGK1 limits NLRP3 inflammasome activation and protects from pyroptosis. | Nature Communications

Fig. 2: SGK1 limits NLRP3 inflammasome activation and protects from pyroptosis.

From: Serum/glucocorticoid-inducible kinase 1 deficiency induces NLRP3 inflammasome activation and autoinflammation of macrophages in a murine endolymphatic hydrops model

Fig. 2

a–d Primary mVRML cells from sgk1+/+ or sgk1−/− mice upon stimulation with LPS (200 ng/mL, 3 h) alone or with ATP (5 mM, 30 min). a Quantitative real-time PCR of indicated genes (n = 3 biologically independent experiments). b Cell lysates were subjected to western blotting. c ELISA showing Casp-1 and IL-1β secretion (n = 3 biologically independent experiments). d Confocal microscopy of NLRP3 (green) and DAPI (blue) staining, scale bar = 50 μm. e–h Control and SGK1 siRNA-transfected THP-1 cells were stimulated with LPS (200 ng/mL, 3 h) alone or with ATP (5 mM, 30 min). e Quantitative real-time PCR of indicated genes (n = 3 biologically independent experiments). f Cell lysates were subjected to western blotting. g ELISA showing Casp-1 and IL-1β secretion (n = 3 biologically independent experiments). h Confocal microscopy of ASC (green) and DAPI (blue) staining, scale bar = 50 μm. i Dot plot showing flow cytometry analysis of control and SGK1 siRNA-transfected THP-1 cells with or without LPS and ATP in the presence or absence of z-VAD-FMK (20 μM, 3.5 h) or Ac-YVAD-cmk (40 μM, 3.5 h). j Western blotting with control and SGK1 siRNA-transfected THP-1 cells with or without LPS and ATP in the presence or absence of z-VAD-FMK (20 μM, 3.5 h) or Ac-YVAD-cmk (40 μM, 3.5 h). Results are presented as mean ± SD. Statistical analyses were carried out via two-sided t-test for a, c, e, g. mVRMLs, mouse vestibular-resident macrophage-like cells; Casp-1 caspase-1. Source data are provided as a Source Data file.

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