Fig. 5: Metabolomics profiling of 3HP-producing A. pseudoterreus and A. niger strains. | Nature Communications

Fig. 5: Metabolomics profiling of 3HP-producing A. pseudoterreus and A. niger strains.

From: PeakDecoder enables machine learning-based metabolite annotation and accurate profiling in multidimensional mass spectrometry measurements

Fig. 5

a Relative and label-free intracellular metabolites levels quantified by PeakDecoder (n = 46). Red, yellow, and blue colors indicate high, medium, and low log2 intensity values, and gray color indicates missing values. b CCS errors of the good-quality features in 24 samples confirmed the detection of 3HP (green bar, 113.8 CCS) instead of lactic acid (orange bar, 113.0 CCS), which is an isomeric molecule (same formula but with different 3D structure). c Metabolites in the 3HP production pathway and their log2 fold changes over the control sample (parent strain). Statistical analysis was performed using the IMD-ANOVA method. Stars indicate statistically significant changes (*p-value <0.05, **p-value <0.01, and ***p-value <0.001). Y-axis for pyruvic (A. pseudoterreus) and 2,4-diaminobutanoic acids represent mean log2 intensity due to no detection in the control strain. Source data are provided as a Source data file.

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