Fig. 2: Resource competition model in the two-reporter system. | Nature Communications

Fig. 2: Resource competition model in the two-reporter system.

From: Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system

Fig. 2

a The genetic circuit design for the RNAP-driven expression of two reporter genes. b Characterization of resource competition by two co-expressed reporters in HEK293T. Experimental data and model predictions are compared at varying activities for the two reporters. Data represent mean values of more than three biological replicates. c Schematic of different promoters competing for the same pool of free RNAP ([RNAP]free). d Heatmap showing the concentration of [RNAP]free as calculated by the biochemical model with resource competition. Promoter activities are decreased as shown by the triangles for two reporter genes. Source data are provided as a Source data file.

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