Fig. 3: Gene-order effect for three-gene expression and predictions of the resource-competition model. | Nature Communications

Fig. 3: Gene-order effect for three-gene expression and predictions of the resource-competition model.

From: Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system

Fig. 3

a Schematic of the three-reporter characterization system with all possible orders of the transcriptional units. b Relative activity of blue, yellow and red fluorescent proteins of each reporter gene (bfp, yfp, and mCherry) based on their respective expression level in construct #1. Data represent the mean ± SD (n = 4). c Schematic of the circuit for the performance evaluation of three-reporter gene expression system. d Three-dimensional graph of the combinatorial promoter library with different binding affinities to the cognate RNAP. Different colors show different z-axis (Pbfp value). e Correlation between predicted promoter activities and experimental fluorescent intensities on a log–log scale for the three reporter genes. Data represent the mean ± SD (n = 3). Source data are provided as a Source data file.

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