Fig. 6: Inhibition of the deISGylase activity of full-length SARS-CoV-2 hemagglutinin (HA)-Nsp3 transiently expressed in HEK293T cells.
From: Potent and selective covalent inhibition of the papain-like protease from SARS-CoV-2
A Anti-HA beads after immunoprecipitation (IP) and whole cell lysates probed with anti-HA antibody. The asterisks indicate immunoglobulin G (IgG) heavy chain (HC) and light chain (LC). Anti-HA beads were assayed for Nsp3 deISGylase activity using an ISG15-CHOP2 assay in the presence of the dose range of B compound 7 or C GRL0617. Data are presented as mean values for n = 2 independent experiments for compound 7 and n = 3 independent samples for GRL0617. Curves are nonlinear regressions to the normalized dose response equation. Source data are provided as a Source Data file.
