Fig. 2: Design and preparation of PikCH238pAcF. | Nature Communications

Fig. 2: Design and preparation of PikCH238pAcF.

From: Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids

Fig. 2

a Overview of the superimposed PikC active sites with productive binding of 4 and 5 (PDB ID codes: 2VZ7 and 2VZM). The heme group and amino acid residues targeted for mutagenesis are displayed as sticks. Substrates 4 and 5 are shown in slate and cyan, respectively. The iron atom is shown as sphere. b SDS-PAGE analysis of purified PikCWT and PikCH238pAcF. Source data are provided as a Source Data file. c ESI-TOF MS analysis of the purified PikCH238pAcF protein. A peak measured at 49187.8187 Da corresponds to PikCH238pAcF lacking the initiating methionine residue (the calculated mass of PikCH238pAcF: 49187.7500 Da).

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