Fig. 1: Cardiomyocyte-specific Mrps5 deletion results in cardiac hypertrophy and heart failure.

a Gene expression level of Mrps5 and other regulators of mitochondrial gene expression and ETC genes are downregulated in hypertrophic hearts. b, c Western blot of protein levels of MRPS5 and mitochondrial encoded protein mt-ATPase 6 in control (Sham) and hypertrophic mouse hearts (TAC). d Gene expression level of Mrps5 and other regulators of mitochondrial gene expression and ETC genes in isolated neonatal mouse cardiomyocytes (NMCMs) in response to hypertrophic agonizts phenylephrine (PE), isoproterenol (ISO) and fetal bovine serum (FBS) in vitro e, f Analysis of protein levels of MRPS5 and mt-ATPase 6 in human hearts from control or patients diagnosed with dilated cardiomyopathy (DCM). g Gene expression level of Mrps5 is significantly decreased at 2 weeks to 18 weeks after tamoxifen injection. h Gross heart morphology from control Mrps5^fl/fl and Mrps5^cKO mice at 12 weeks after tamoxifen injection. Scale bar = 500 µm. i Heart sections stained with hematoxylin and eosin (top panel) or Sirius Red and Fast Green (bottom panel) from control Mrps5^fl/fl and Mrps5^cKO mice at 12 weeks after tamoxifen injection. Scale bar = 500 µm. j Heart weight to body weight ratio of Mrps5^fl/fl and Mrps5^cKO mice at 2 to 22 weeks after tamoxifen injection. k Representative images of heart cross sections from Mrps5^fl/fl and Mrps5^cKO mice at 8, 12, and 18 weeks after tamoxifen injection (immunostained with Wheat germ agglutinin (WGA) in red and DAPI in blue). Scale bar = 100 μm. l Quantification of the cross-sectional area of cardiomyocytes and m Cardiac fibrosis from Mrps5^fl/fl and Mrps5^cKO mice at 4 to 18 weeks after tamoxifen injection. n Survival curve of Mrps5^fl/fl and Mrps5^cKO mice post tamoxifen injection. o Echocardiographic measurement of cardiac function in Mrps5^fl/fl and Mrps5^cKO mice at 5 to 23 weeks after tamoxifen injection. p qRT-PCR analysis of cardiomyopathy marker genes from control Mrps5^fl/fl and Mrps5^cKO mouse hearts at 6 to 18 weeks after tamoxifen injection. N numbers are indicated in each panel. All data were presented as mean ± SEM. P values were determined by a two-tailed unpaired Students’ t-test.