Fig. 3: PEMP display characteristics of primitive erythro-myeloid progenitors. | Nature Communications

Fig. 3: PEMP display characteristics of primitive erythro-myeloid progenitors.

From: Primitive haematopoiesis in the human placenta gives rise to macrophages with epigenetically silenced HLA-DR

Fig. 3

A Schematic representation of human haematopoietic progenitor populations used for comparative analysis25,26,27,29. CS Carnegie Stage, EMP Erythro-myeloid progenitors, YSMP Yolk sac myeloid-biased progenitors, AGM Aorta-gonad mesonephros, FL Foetal liver, BM Bone marrow, HSC Haematopoietic stem cell. B Hierarchical clustering dendrogram depicting transcriptomic similarity between human foetal progenitor populations, calculated using gene expression distance matrix from the integrated dataset (Fig. S7A). C Scatter plot showing differentially expressed genes (DEGs) between PEMP-1 and definitive progenitors (YSMP, AGM HSC, FL HSC and BM HSC) on the x-axis and CS7 EMP and definitive progenitors on the y-axis. Genes significantly differentially expressed (adjusted p value <0.05) in both comparisons, PEMP-1 alone and CS7 EMP alone are shown in purple, orange and blue respectively. Selected genes which were differentially expressed in both CS7 EMP and PEMP-1 are highlighted. D Violin plot of HLF log-normalised gene expression across human foetal haematopoietic progenitors. E Hierarchical clustering dendrogram depicting transcriptomic similarity between human and murine37,38 foetal progenitor populations, calculated using gene expression distance matrix from the integrated dataset (Fig. S7C). YS – Yolk sac. F Venn diagram depicting the overlap of DEGs calculated between all human and all murine foetal haematopoietic progenitors, and DEGs calculated between PEMP-1 and murine placental HSC alone. G Volcano plots showing DEGs between PEMP-1 and murine placental HSC. Left panel: genes which overlap with DEGs calculated between all human and all murine foetal haematopoietic progenitors. Right panel: genes which do not overlap. H Violin plot of Hlf log-normalised gene expression across murine foetal haematopoietic progenitors.

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