Fig. 1: The colocalization of PTBP1 with endothelial cells during heart development.
From: Endothelial deletion of PTBP1 disrupts ventricular chamber development
a Immunofluorescence staining of PTBP1 (cyan) with the cardiomyocyte marker cTnT (red) in embryonic hearts. The magnified images indicate the distribution pattern of PTBP1 in ventricular tissues. The expression of PTBP1 is almost undetectable in cardiomyocytes. b Immunofluorescence staining of PTBP1 in Tie2-Cre;Rosa26-tdTomato embryonic hearts. The magnified images indicate the colocalization of PTBP1 (cyan) with tdTomato-marked endothelial cells (red) in Tie2-Cre;Rosa26-tdTomato ventricles. The white arrowheads indicate the colocalization of PTBP1 with endocardial cells; the yellow arrowheads indicate the colocalization of PTBP1 with endothelial cells in the compact myocardium. Scale bars in E9.5 and E11.5 images are 200 µm; Scale bars in E13.5 and E15.5 images are 400 µm; Scale bars in the zoomed images are 50 µm. A atrium, V ventricle, LV left ventricle, RV right ventricle.
