Fig. 5: SIRT2 mediates the hypothalamic actions of eNAMPT. | Nature Communications

Fig. 5: SIRT2 mediates the hypothalamic actions of eNAMPT.

From: Circulating blood eNAMPT drives the circadian rhythms in locomotor activity and energy expenditure

Fig. 5

a Circadian patterns of the expression of SIRT1 and SIRT2 in the MBH of normal mice (ZT10, 22, n = 2; other groups, n = 3 mice). Two independent replicates were performed. ZT zeitgeber time. b Representative images of Sirt2 fluorescent in situ hybridization and POMC (ß-endorphin) or NPY-GFP immunofluorescence double staining showing Sirt2 expression in the POMC and NPY/AgRP neurons of the hypothalamic arcuate nucleus (ARC). Two independent replicates were performed. Scale bars = 25 μm and 10 μm (magnified images). c Effects of NAMPT/NMN treatment on POMC and AGRP promoter activities in SH-SY5Y cells with or without SIRT2 knockdown (NAMPT, n = 5; MNM, n = 3 cell wells). One-way ANOVA followed by Fisher’s LSD test. n.s.: not significant. Three independent experiments were performed to validate the results. d Schematic diagram of the hypothalamic Sirt2 depletion study. Some animals were subjected to killing study without CLAMS. e, f Effect of hypothalamic Sirt2 depletion with bilateral ARC injection of siSirt2 on i.c.v. NAMPT (100 ng)-induced changes in LMA, EE, and hypothalamic neuropeptide expression in mice (LMA/EE, n = 4; neuropeptide, n = 5 mice). One-way ANOVA followed by Fisher’s LSD test. Two independent replicates were performed. Data are presented as mean ± SEM.

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