Fig. 1: Profiling of regulatory element interactome in PCa cell lines.

a Overview of HiChIP experiments and analyses in PCa cell lines. b The number of significant loops (FDR < 0.05) in VCaP HiChIP assays. From top to bottom, n = 74514, 61748, 46819, 9751, 24982, 24737, respectively. c Strength of H3K27ac and AR loops anchored at TSSs of all protein-coding genes. The frequency curves showed the normalized read number (loop strength) distribution at anchors distal to TSSs. The strength of each loop was normalized by the number of total ‘cis-far’ unique valid pairs. P-values were determined by paired t-test. For TSSs, n = 19962. d Strength of H3K27ac loops anchored at genes downregulated or upregulated by 2 h DHT treatment. The bar plot summarized the strength of loops-anchored gene TSSs. P-values were determined by paired t-test. For TSSs of upregulated and downregulated genes, n = 350 and 205, respectively. e Strength of AR loops anchored at genes downregulated or upregulated by 2 h DHT treatment. The bar plot summarized the strength of loops anchored at gene TSSs. P-values were determined by paired t-test. For TSSs of upregulated and downregulated genes, n = 350 and 205, respectively. f The expression of IL20RA was upregulated from the early time point (2 h) after DHT stimulation. n = 2. Data represent means ± SD. g For the IL20RA gene, AR loops were boosted as early as 2 h after DHT stimulation. h 3C–qPCR assay of the IL20RA genomic region. The data represents relative frequencies of interaction between the anchor region near the IL20RA TSS and selected PstI digestion sites (circles). n = 3. Data represent means ± SD. P-values were determined by Student’s t-test. *P < 0.05; **P < 0.01. Source data are provided as a Source Data file.