Fig. 3: Frequency of Spike-specific T_RM cells in the lung.

a Representative flow-cytometry plots showing three subsets of CD4⁺ T cells present in the lung: CD69^- non-T_RM, CD69⁺ T_RM, and CD69⁺CD103⁺ T_RM cells expressing CD107a and IFNγ after exposure of single-cell suspensions of lung tissue to S-peptide pools or left unstimulated for an Inf and an LT patient. b Comparison of the net frequency of S-specific IFNγ⁺ cells within the three (non-) T_RM cell subsets present in the lung for each group. Data in bar graphs are shown as median ± IQR, where each dot represents an individual patient for each group (Ctrl, control, n = 5; Inf, convalescent infected, n = 8; LT, vaccine 2/3 doses, n = 10, and ST, vaccine 3/4 doses, n = 5). Statistical significance was determined by the Friedmann test (with Dunn´s post-test, two-sided) for the difference between the cellular subsets within each patient group. Source data are provided as a Source Data file.