Fig. 1: The tumor-promoting effect of AA on CRC tumorigenesis. | Nature Communications

Fig. 1: The tumor-promoting effect of AA on CRC tumorigenesis.

From: FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer

Fig. 1

a AA levels in paired NC, Ad and CRC samples, NC: normal intestinal epithelial tissues, Ad: colon adenoma, CRC: colorectal cancer. ***p < 0.001. (n = 15 per group, means ± s.d.; two-tailed paired t test, n = 3 technical replicates). b CRC model established by 10 mg/kg of AOM induction (i.p injection) and 2% of DSS in drinking water using C57BL/6 J mice, NC: normal feeding, AA: arachidonic acid feeding (n = 8 mice per group). c Representative images of AOM/DSS-induced tumor in full-length colons (n = 8 mice per group). Black circles showed the tumor region. d Tumor number of AOM/DSS mice for NC and AA groups (n = 8 mice per group, means ± s.d.; two-tailed unpaired t test, n = 2 biological replicates), **p = 0.003. e HE staining and PCNA expression in AOM/DSS tumors (n = 8 samples per group, 3 fields assessed per sample, Chi-square test). Scale bars, 50 μm. Tumor grade was independently assessed by two staff members of the pathology department, low grade and high grade represents low and high-grade intraepithelial neoplasia. ***p < 0.001. f AA and prostaglandins levels in tumor tissues in NC and AA groups in AOM/DSS-induced mice (n = 8 samples per group, means ± s.d., n = 3 technical replicates). Liquid chromatography-mass spectrometry (LCMS) was used to detect the index levels. g Intestine-specific Apc−/− mice generated by crossing Apcflox/flox with Lgr5-EGFP-IRES-CreERT2 mice, NC: normal feeding, AA: arachidonic acid feeding (n = 6 mice per group). h Representative images of CRC tumors in intestine-specific Apc−/− mice (n = 6 mice per group). Black circles showed the tumor region. i. Tumor number of intestine-specific Apc−/− mice for NC and AA groups (n = 6 mice per group; means ± s.d., two-tailed unpaired t test, n = 2 biological replicates). ***p < 0.001. j HE staining and PCNA expression in tumor tissues of intestine-specific Apc−/− mice (n = 6 samples per group, 3 fields assessed per sample, Chi-square test). Tumor grade was independently assessed. ***p < 0.001. Scale bars, 50 μm. k AA and prostaglandins levels in tumor tissues of intestine-specific Apc−/− mice. LCMS was used to detect the index levels. (n = 6 samples per group, means ± s.d.). Source data are provided in the Source Data file.

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