Fig. 2: Gut microbes mediate tumor promotion via AA. | Nature Communications

Fig. 2: Gut microbes mediate tumor promotion via AA.

From: FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer

Fig. 2

a The viability of SW480 and HCT-116 cells treated with different concentration (NC, 0.5 mg/L, 1 mg/L, 2 mg/L) of AA, and analyzed by CCK-8. ns indicates no statistical significance (n = 5 samples per group; means ± s.d., one-way repeated-measures ANOVA, n = 3 biological replicates). b EdU assay for SW480 and HCT-116 cells treated with different concentrations (NC, 0.5 mg/L, 1 mg/L, 2 mg/L) of AA (n = 5 samples per group, 3 fields assessed per sample, means ± s.d., two-tailed unpaired t test). ns indicates no statistical significance. Scale bars, 50 μm. c. 16 s rRNA sequencing in stool of C57BL6J mice divided into NC and AA groups, NC: normal feeding, AA: arachidonic acid feeding (n = 8 mice per group). d Total OTU of gut microbes in the stool of C57BL6J mice, NC: normal feeding, AA: arachidonic acid feeding (n = 8 mice per group, means ± s.d., two-tailed unpaired t test, n = 3 technical replicates). ns indicates no statistical significance. e Ratio of gram-negative bacteria to gram-positive bacteria in the stool of C57BL6J mice in NC and AA groups (n = 8 mice per group, Chi square test). *p = 0.034. f Relative abundance of bacterial OTUs in the stool of C57BL6J mice in NC and AA groups (n = 8 mice per group). g Relative abundance of Es. coli, En. faecalis and Lactobacillus in the stool and tumor tissue of AOM/DSS mice in NC and AA groups (n = 8 mice per group, means ± s.d., two-tailed unpaired t test, n = 3 technical replicates). *p = 0.016, 0.039, 0.018, 0.041, 0.012, 0.045. h Representative images of AOM/DSS tumors with NC or AA feeding under different antibiotics treatment (Quadruple-antibiotics, 0.2 g/L of aztreonam, 0.1 g/L of vancomycin) (n = 6 mice per group). Black circles showed tumor region. i Tumor number of AOM/DSS-induced mice with NC or AA feeding under different antibiotics treatment (n = 6 mice per group; means ± s.d., two-tailed unpaired t test). ***p < 0.001(compared with NC group). j HE staining and PCNA expression in AOM-induced tumors with NC or AA feeding under different antibiotics treatment (n = 6 samples per group, 3 fields assessed per sample). Scale bars, 50 μm. Source data are provided in the Source Data file.

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