Fig. 3: FADS1 is upregulated in CRC and controls AA synthesis. | Nature Communications

Fig. 3: FADS1 is upregulated in CRC and controls AA synthesis.

From: FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer

Fig. 3

a The mRNA expression of FADS1, FADS2, FADS3, ELOVL2, and ELOVL5 in 17 cases of paired NC and CRC samples from GDS4382, NC: normal colon epithelial tissue, CRC: colorectal cancer. ***p < 0.001. ns indicates no statistical significance. b EPA levels in 15 cases of paired NC, Ad and CRC samples. ns indicates no statistical significance. (n = 15 samples per group, means ± s.d.; two-tailed paired t test, n = 3 technical replicates). c. Correlation between FADS1 expression and AA or EPA levels in 15 cases of paired NC, Ad and CRC samples. The mRNA expression of FADS1 was detected by q-PCR. r indicates correlation coefficient. p value indicates statistical difference (n = 15 per group, means ± s.d.; Pearson correlation test, n = 3 technical replicates). d Total AA and EPA levels in SW480 and HCT116 cells transfected with shNC or shFADS1. ***p < 0.001. ns indicates no statistical significance (n = 3 samples per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). e AA/EPA ratio in SW480 and HCT116 cells transfected with shNC or shFADS1 (n = 3 biological replicates). f. AA levels in culture medium from SW480 and HCT116 cells transfected with shNC or shFADS1, under activation with 0.5 μg/ml and 1 μg/ml of LPS, respectively (n = 3 samples per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). CRC cells were cultured with FBS-free medium before LPS activation. ***p < 0.001. g EPA levels in culture medium of SW480 and HCT116 cells transfected with shNC or shFADS1, under activation with 0.5 μg/ml and 1 μg/ml of LPS, respectively (n = 3 samples per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). CRC cells were cultured with FBS-free medium before LPS activation. ***p < 0.001. h AA/EPA ratio in culture medium of SW480 and HCT116 cells transfected with shNC or shFADS1, under activation with 0.5 μg/ml and 1 μg/ml of LPS, respectively (n = 3 biological replicates). CRC cells were cultured with FBS-free medium before LPS activation. Source data are provided in the Source Data file.

Back to article page