Fig. 6: The TLR4/MYD88 pathway is involved in the gut microbe-mediated PGE2 production.

a PGE2 levels in SW480 and HCT116 cells transfected with siTLR4, activated by 1 μg/ml of LPS (n = 3 per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). ***p < 0.001. b PGE2 level in SW480 and HCT116 cells transfected with siMYD88, activated by 1 μg/ml of LPS (n = 3 per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). ***p < 0.001. c TLR4 and MYD88 expression in SW480 and HCT116 cells transfected with shFADS1 or shNC. TLR4 and MYD88 expression were detected by q-PCR and western blot. The grayscale values of the WB bands are quantitatively represented (n = 3 biological replicates). d The mRNA expression of PTGES and PTGS2 in SW480 and HCT116 cells transfected with siTLR4 and siMYD88, activated by 1 μg/ml of LPS (n = 3 per group, means ± s.d.; two-tailed unpaired t test, n = 3 biological replicates). ***p < 0.001. e The protein expression of PTGES and PTGS2 in SW480 and HCT116 cells transfected with siTLR4 and siMYD88, activated by 1 μg/ml of LPS. The grayscale values of the WB bands are quantitatively represented (n = 3 biological replicates). f Viability of SW480 and HCT116 cells transfected with siTLR4, siMYD88, siPTGES, siPTGS2 and siNC, activated by 1 μg/ml of LPS, rescued by adding 1 mg/L of PGE2 (n = 5 samples per group; means ± s.d., one-way repeated-measures ANOVA, n = 3 biological replicates). ***p < 0.001, ns indicates no statistical significance. Source data are provided in the Source Data file.