Fig. 2: Protein expression analysis at 24 h after mRNA transfection in VERO E6 cells. | Nature Communications

Fig. 2: Protein expression analysis at 24 h after mRNA transfection in VERO E6 cells.

From: Immunogenicity and protective efficacy of SARS-CoV-2 mRNA vaccine encoding secreted non-stabilized spike in female mice

Fig. 2

a Intracellular S protein expression examined by immunofluorescent assay employing anti-RBD, -S1, -S2 or PCS as primary antibody, the nuclei were counter stained with DAPI (blue). FITC-tagged 2nd Abs (green) were used for detection of RBD, S1, and S2 while AlexaFluor647-tagged 2nd Ab (red) was used following PCS staining. b hACE-2 binding assay (merged): culture supernatant collected from ChulaCov19 transfected cells incubated with HEK293T- hACE-2 cells. S protein on HEK293T-hACE-2 cell surface was stained with the same antibodies used in 2a. c S protein expression in cell culture supernatant analyzed by western blot using anti-RBD, -S1, -S2 or PCS as primary antibody. Recombinant S protein with abolished S1/S2 cleavage site was used as positive control in HEK293T-hACE-2 binding assay (right panel of 2b) and western blot (right lane of each panel in 2c). S0 was used to depict unprocessed S protein. Experiments were repeated two times independently with similar results.

Back to article page